Ford-Knight DHCR7: EMBL-EBI IntEnz

Ford-Knight Research: DHCR7

To learn about the information provided by specific pages, click on the link below. (To return to the homepage, click title bar) EMBL-EBI IntEnz Ensembl Protein Report KEGG Author: Karlina Merkens Last Modified: 10 December 2003	EMBL-EBI IntEnz (concise and neat, but very basic info, links to brenda, wit, expacy, kegg) http://www.ebi.ac.uk/intenz/query?cmd=SearchEC&ec=1.3.1.21 This link will bring up the EMBL-EBI IntEnz page with information on DHCR7. To best utilize the information below, please open the linked page and use my directions to learn what information is available there. The EMBL-EBI page is run by the European Bioinformatics Institute. IntEnz is the Integrated Enzyme database, a database within EMBL-EBI, which contains information about enzymes that have been listed in at least one of three different European databases (NC-ICBMB Enzyme Classification list, ENZYME enzyme nomenclature database, and BRENDA functional database.) IntEnz compiles the information from these three pages creating a database that is organized according to function of the enzymes (kind of an enzyme phylogeny). An EMBL-EBI IntEnz page has basic information that could be a good starting point for research. How to read the EMBI-EBI IntEnz page How to search for an enzyme in the EMBL-EBI INTENZ database How to read an EMBL-EBI IntEnz Page: The top half of the page gives you the classification of the protein you have looked up. In the case of DHCR7 that section looks like this: Every protein classified in the IntEnz web site has a four-number EC number that is specific to that single enzyme. (EC stands for Enzyme Classification, and this code is used commonly in European databases.) The above list gives the descriptions of the four classification groups that DHCR7 is part of. The top line ("EC 1") is the broadest classification. The groupings becoming more selective until you reach the bottom line (green box "EC 1.3.1.21") which names only the single protein that the page is for. The words to the left of the bottom EC number is the name of that enzyme. All of these groupings are made according to the function of the enzyme, so all enzymes with an EC number that begins with "1" will be oxidoreductases, all enzymes with an EC number beginning with "1.3" will act on the CH-CH groups of donors, and so on. The bottom half of the EMML-EBI report page provides you with the common name of the enzyme, the reaction that the enzyme is part of, the systematic name (a name that essentially combines the common name and the reaction that it is part of), links to other databases, and one or more key articles that focus on that enzyme. The links to other databases will take you directly to the page in that database for the enzyme that the IntEnz page is about (in this case DHCR7). back to top How to search for an enzyme in the EMBL-EBI IntEnz Web site: To search for your enzyme in the IntEnz database follow this link to the IntEnz Homepage, scroll part way down the page until you find the search box, enter the name or EC number of your enzyme, and click the search button. If you wish to browse the IntEnz database follow the same link and scroll down just a little way until you see the list of six different large group categories (EC 1 Oxidoreductases, EC 2 Transferases, EC 3 Hydrolases, EC 4 Lyases, EC 5 Isomerases, EC 6 Ligases). The "EC #" is linked to the page for that group. images courtesy of EMBL-EBI IntEnz back to top

EMBL-EBI IntEnz
(concise and neat, but very basic info, links to brenda, wit, expacy, kegg)

http://www.ebi.ac.uk/intenz/query?cmd=SearchEC&ec=1.3.1.21
This link will bring up the EMBL-EBI IntEnz page with information on DHCR7. To best utilize the information below, please open the linked page and use my directions to learn what information is available there.

The EMBL-EBI page is run by the European Bioinformatics Institute. IntEnz is the Integrated Enzyme database, a database within EMBL-EBI, which contains information about enzymes that have been listed in at least one of three different European databases (NC-ICBMB Enzyme Classification list, ENZYME enzyme nomenclature database, and BRENDA functional database.) IntEnz compiles the information from these three pages creating a database that is organized according to function of the enzymes (kind of an enzyme phylogeny). An EMBL-EBI IntEnz page has basic information that could be a good starting point for research.

The top half of the page gives you the classification of the protein you have looked up. In the case of DHCR7 that section looks like this:

Every protein classified in the IntEnz web site has a four-number EC number that is specific to that single enzyme. (EC stands for Enzyme Classification, and this code is used commonly in European databases.) The above list gives the descriptions of the four classification groups that DHCR7 is part of. The top line ("EC 1") is the broadest classification. The groupings becoming more selective until you reach the bottom line (green box "EC 1.3.1.21") which names only the single protein that the page is for. The words to the left of the bottom EC number is the name of that enzyme. All of these groupings are made according to the function of the enzyme, so all enzymes with an EC number that begins with "1" will be oxidoreductases, all enzymes with an EC number beginning with "1.3" will act on the CH-CH groups of donors, and so on.

The bottom half of the EMML-EBI report page provides you with the common name of the enzyme, the reaction that the enzyme is part of, the systematic name (a name that essentially combines the common name and the reaction that it is part of), links to other databases, and one or more key articles that focus on that enzyme. The links to other databases will take you directly to the page in that database for the enzyme that the IntEnz page is about (in this case DHCR7).

To search for your enzyme in the IntEnz database follow this link to the IntEnz Homepage, scroll part way down the page until you find the search box, enter the name or EC number of your enzyme, and click the search button. If you wish to browse the IntEnz database follow the same link and scroll down just a little way until you see the list of six different large group categories (EC 1 Oxidoreductases, EC 2 Transferases, EC 3 Hydrolases, EC 4 Lyases, EC 5 Isomerases, EC 6 Ligases). The "EC #" is linked to the page for that group.